DNA repair enables sex identification in genetic material from human teeth
Identifieur interne : 000916 ( Main/Exploration ); précédent : 000915; suivant : 000917DNA repair enables sex identification in genetic material from human teeth
Auteurs : L. Kovatsi [Grèce] ; D. Nikou [Grèce] ; S. Triantaphyllou [Royaume-Uni] ; S N Njau [Grèce] ; S. Voutsaki [Pays-Bas] ; S. Kouidou [Grèce]Source :
- Hippokratia [ 1108-4189 ] ; 2009.
Abstract
Url:
PubMed: 19918305
PubMed Central: 2765294
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000287
- to stream Pmc, to step Curation: 000287
- to stream Pmc, to step Checkpoint: 000206
- to stream Ncbi, to step Merge: 000983
- to stream Ncbi, to step Curation: 000983
- to stream Ncbi, to step Checkpoint: 000983
- to stream Main, to step Merge: 000928
- to stream Main, to step Curation: 000916
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">DNA repair enables sex identification in genetic material from human teeth</title>
<author><name sortKey="Kovatsi, L" sort="Kovatsi, L" uniqKey="Kovatsi L" first="L" last="Kovatsi">L. Kovatsi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki</wicri:regionArea>
<wicri:noRegion>Aristotle University of Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nikou, D" sort="Nikou, D" uniqKey="Nikou D" first="D" last="Nikou">D. Nikou</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki</wicri:regionArea>
<wicri:noRegion>Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Triantaphyllou, S" sort="Triantaphyllou, S" uniqKey="Triantaphyllou S" first="S" last="Triantaphyllou">S. Triantaphyllou</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Sheffield Centre for Aegean Archaeology, Department of Archaeology, University of Sheffield, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Sheffield Centre for Aegean Archaeology, Department of Archaeology, University of Sheffield</wicri:regionArea>
<wicri:noRegion>University of Sheffield</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Njau, S N" sort="Njau, S N" uniqKey="Njau S" first="S N" last="Njau">S N Njau</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki</wicri:regionArea>
<wicri:noRegion>Aristotle University of Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Voutsaki, S" sort="Voutsaki, S" uniqKey="Voutsaki S" first="S" last="Voutsaki">S. Voutsaki</name>
<affiliation wicri:level="4"><nlm:aff id="A4">Groningen Institute of Archaeology, University of Groningen, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Groningen Institute of Archaeology, University of Groningen</wicri:regionArea>
<placeName><settlement type="city">Groningue (ville)</settlement>
<region>Groningue (province)</region>
</placeName>
<orgName type="university">Université de Groningue</orgName>
</affiliation>
</author>
<author><name sortKey="Kouidou, S" sort="Kouidou, S" uniqKey="Kouidou S" first="S" last="Kouidou">S. Kouidou</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki</wicri:regionArea>
<wicri:noRegion>Thessaloniki</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19918305</idno>
<idno type="pmc">2765294</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2765294</idno>
<idno type="RBID">PMC:2765294</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">000287</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000287</idno>
<idno type="wicri:Area/Pmc/Curation">000287</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000287</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000206</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000206</idno>
<idno type="wicri:Area/Ncbi/Merge">000983</idno>
<idno type="wicri:Area/Ncbi/Curation">000983</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000983</idno>
<idno type="wicri:doubleKey">1108-4189:2009:Kovatsi L:dna:repair:enables</idno>
<idno type="wicri:Area/Main/Merge">000928</idno>
<idno type="wicri:Area/Main/Curation">000916</idno>
<idno type="wicri:Area/Main/Exploration">000916</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">DNA repair enables sex identification in genetic material from human teeth</title>
<author><name sortKey="Kovatsi, L" sort="Kovatsi, L" uniqKey="Kovatsi L" first="L" last="Kovatsi">L. Kovatsi</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki</wicri:regionArea>
<wicri:noRegion>Aristotle University of Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nikou, D" sort="Nikou, D" uniqKey="Nikou D" first="D" last="Nikou">D. Nikou</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki</wicri:regionArea>
<wicri:noRegion>Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Triantaphyllou, S" sort="Triantaphyllou, S" uniqKey="Triantaphyllou S" first="S" last="Triantaphyllou">S. Triantaphyllou</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Sheffield Centre for Aegean Archaeology, Department of Archaeology, University of Sheffield, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Sheffield Centre for Aegean Archaeology, Department of Archaeology, University of Sheffield</wicri:regionArea>
<wicri:noRegion>University of Sheffield</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Njau, S N" sort="Njau, S N" uniqKey="Njau S" first="S N" last="Njau">S N Njau</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki</wicri:regionArea>
<wicri:noRegion>Aristotle University of Thessaloniki</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Voutsaki, S" sort="Voutsaki, S" uniqKey="Voutsaki S" first="S" last="Voutsaki">S. Voutsaki</name>
<affiliation wicri:level="4"><nlm:aff id="A4">Groningen Institute of Archaeology, University of Groningen, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Groningen Institute of Archaeology, University of Groningen</wicri:regionArea>
<placeName><settlement type="city">Groningue (ville)</settlement>
<region>Groningue (province)</region>
</placeName>
<orgName type="university">Université de Groningue</orgName>
</affiliation>
</author>
<author><name sortKey="Kouidou, S" sort="Kouidou, S" uniqKey="Kouidou S" first="S" last="Kouidou">S. Kouidou</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki, Greece</nlm:aff>
<country xml:lang="fr">Grèce</country>
<wicri:regionArea>Laboratory of Biological Chemistry, School of Medicine, Aristotle University, Thessaloniki</wicri:regionArea>
<wicri:noRegion>Thessaloniki</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Hippokratia</title>
<idno type="ISSN">1108-4189</idno>
<idno type="eISSN">1790-8019</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><bold>Background:</bold>
The purpose of this study was to test the effectiveness of a DNA repair protocol in improving genetic testing in compromised samples, frequently encountered in Forensic Medicine.</p>
<p><bold>Methods:</bold>
In order to stretch the experiment conditions to the limits, as far as quality of samples and DNA is concerned, we tried the repair protocol on ten ancient human teeth obtained from an equal number of skeletons from a burial site in Lerna, Middle Helladic Greece (2100 - 1700 BC). For these samples, sex was previously determined morphologically, serving as a reference to compare our molecular data with. The samples were analysed using the DNA amelogenin sex test assay prior and after DNA polymerase repair. For every individual, two molecular sex determinations were obtained by visualising PCR products on an agarose gel.</p>
<p><bold>Results:</bold>
DNA repair enabled genetic testing in these samples. Successful amplification of the amelogenin gene was obtained only from the repaired DNA in eight out of ten samples. Prior to the repair treatment, none of these samples yielded any PCR products, thus attesting to the authenticity of the amplified sequence. The concordance between morphological and molecular analysis was in reasonable agreement (71%).</p>
<p><bold>Conclusions:</bold>
These results reveal the impact of the repair process in studying single copy genes from low quality DNA. This protocol could facilitate molecular analysis in compromised samples, encountered in forensic medicine, as well as enable genetic studies in ancient remnants.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Grèce</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
</country>
<region><li>Groningue (province)</li>
</region>
<settlement><li>Groningue (ville)</li>
</settlement>
<orgName><li>Université de Groningue</li>
</orgName>
</list>
<tree><country name="Grèce"><noRegion><name sortKey="Kovatsi, L" sort="Kovatsi, L" uniqKey="Kovatsi L" first="L" last="Kovatsi">L. Kovatsi</name>
</noRegion>
<name sortKey="Kouidou, S" sort="Kouidou, S" uniqKey="Kouidou S" first="S" last="Kouidou">S. Kouidou</name>
<name sortKey="Nikou, D" sort="Nikou, D" uniqKey="Nikou D" first="D" last="Nikou">D. Nikou</name>
<name sortKey="Njau, S N" sort="Njau, S N" uniqKey="Njau S" first="S N" last="Njau">S N Njau</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Triantaphyllou, S" sort="Triantaphyllou, S" uniqKey="Triantaphyllou S" first="S" last="Triantaphyllou">S. Triantaphyllou</name>
</noRegion>
</country>
<country name="Pays-Bas"><region name="Groningue (province)"><name sortKey="Voutsaki, S" sort="Voutsaki, S" uniqKey="Voutsaki S" first="S" last="Voutsaki">S. Voutsaki</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Archeologie/explor/PaleopathV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000916 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000916 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Archeologie |area= PaleopathV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:2765294 |texte= DNA repair enables sex identification in genetic material from human teeth }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19918305" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a PaleopathV1
This area was generated with Dilib version V0.6.27. |